Composite

Part:BBa_K2032000:Design

Designed by: Jacob Premo, Seth Cohen, Chun Mun Loke   Group: iGEM16_UMaryland   (2016-08-27)


sMMO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3102
    Illegal BamHI site found at 3136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 110
    Illegal NgoMIV site found at 1021
    Illegal NgoMIV site found at 1199
    Illegal NgoMIV site found at 3749
    Illegal NgoMIV site found at 4585
    Illegal AgeI site found at 3996
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1160
    Illegal BsaI site found at 1225
    Illegal BsaI.rc site found at 1268
    Illegal BsaI.rc site found at 1724
    Illegal BsaI.rc site found at 2111
    Illegal BsaI.rc site found at 2249
    Illegal BsaI.rc site found at 2666
    Illegal BsaI.rc site found at 3815


Design Notes

A second terminator and promoter to divide the transcript into was considered to avoid potential complications with a long mRNA.


Source

Methylococcus capsulatus, a methanotrophic bacteria

References